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________LABORATORIO________
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ESAME
Ab anti-dengue IgM/IgG
Metodo ELISA
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La dengue e causata da un flavivirus, un arbovirus causa di infezioni umane. Questi virus, dei quali ci sono quattro differenti sierotipi, sono diffusi nelle zone tropicali e subtropicali. La trasmissione avviene attraverso la puntura di una zanzara infetta, Aedes aegypti.
L’infezione e caratterizzata da febbre improvvisa, forte mal di testa, mialgia, artralgia ed eruzioni cutanee.
Gravi complicanze dell'infezione sono dovute alla febbre emorragica da dengue e dalla sindrome da shock dengue; spesso queste complicanze sono il risultato dell'acquisizione da parte dell’individuo di una seconda infezione con un sierotipo diverso.
Nel nostro laboratorio viene eseguita la ricerca degli anticorpi IgM e IgG contro i quattro sierotipi del virus della dengue mediante metodo ELISA.
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Ab anti dengue IgM/IgG (ELISA) (2 esami)
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Codici del Catalogo Veneto Prescrivibile (CVP), prestazioni e tariffario
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Prelievo ematico e/o
consegna campioni
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30 giorni lavorativi
N.B. Non comprende il tempo di arrivo del campione in laboratorio e dell'eventuale invio del referto a domicilio.
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ELISA (Enzyme-Linked ImmunoSorbent Assay)
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| Bibliografia
IgM
| | | - | U.S. Department of Health and Human Services: Public Health Service, Centers for Disease Control and Prevention, National Institutes of Health. (1999). p. 8-16. In (ed.) Richmond JY, McKinney RW, Guidelines: Biosafety in microbiological and biomedical laboratories. 4th Edition. US Government Printing Office, Washington, D.C. | | - | Xu, H., Lohr, J. and Greiner, M. (1997). The selection of ELISA cutoff points for testing antibody to Newcastle disease by two-graph receiver
operating characteristic (TGGROC) analysis. J Immunol. Meth. 208(1):61-64. | | - | Greiner, M., Sohr, D. and Gobel, P. (1995). A modified ROC analysis for selection of cutoff values and the definition of intermediate results in serodiagnostic tests. J Immunol. Meth. 185: 123-132. | | - | Seth, J. (1991). Standardisation and Quality Assurance. In: Principle and Practice of Immunoassay, Price, C.P. and Newman, OJ (Eds). MacMiliian, London. |
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| IgG | | | - | World Health Organisation. (1997). Dengue Haemorrhagic Fever: diagnosis, treatment, prevention and control, p34-36. 2nd edition. World Health Organisation Offie of Publications Geneva, Switzerland. | | - | U.S. Department of Health and Human Services: Public Health Service, Centers for Disease Control and Prevention, National Institutes of Health. (1999). p. 8-16. In (ed.) Richmond JY, McKinney RW, Guidelines: Biosafety in microbiological and biomedical laboratories. 4th Edition. U.S. Government Printing Office, Washington, D.C. | | - | Cuzzubbo, A., et al. (1997). Commercial Assays for the Serological Diagnosis of Dengue Infection. Arbovirus Research in Australia. 7:56-60. Queensland
Institute of Medical Research. | | - | Lam, SK and Devine, P.L. (1998). Evaluation of Capture ELISA and Rapid Immunochromatographic Test for the Determination of IgM and IgG Antibodies Produced During Dengue Infection. Clinical and Diagnostic Virology. 1 0:"15-81. | | - | Innis, B. L., et al. (1989). An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate. Am J Trop Med Hyg. 40:418-427. | | - | Ruechusatawat, K., et al. (1994). Daily observation of antibody levels among dengue patients detected by ELISA. Jap. J. Trop Med. Hyg 22:9. | | - | Makino, Y., et al. (1994). Studies on serological cross-reaction in sequential flavivirus infections. Microbiol. Immunol. 38:951-955. |
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Coproparassitologico, Colorazione protozoi intestinali, Scotch test, Cryptosporidium, Uroparassitologico, Malaria QBC, Malaria emoscopia, Leishmania in midollo, Trypanosoma MHT, Trypanosoma QBC, Leishmania in cute, Microfilarie, Skin snip, Parassiti delle vie respiratorie, Antigene malaria, Antigene Giardia, Antigene Schistosoma, Antigene dengue, Coprocoltura per Strongyloides stercoralis, Identificazione parassita in tessuto, Identificazione ecto ed endoparassiti.
qPCR Strongyloides stercoralis, qPCR Schistosoma, qPCR Triplex EhEdC, qPCR Triplex DGB, qPCR Triplex StScH, qPCR Babesia, qPCR Leishmania, qPCR Trypanosoma cruzi,
Ab anti-malaria, Ab anti-Leishmania, Ab anti-Trypanosoma b. gambiense, Ab anti-Trypanosoma cruzi AgLIs, Ab anti-Trypanosoma cruzi AgRic, Ab anti-Trypanosoma cruzi ICT, Ab anti-filaria, Ab anti-ameba, Ab anti-Strongyloides stercoralis IFAT, Ab anti-Strongyloides stercoralis ELISA, Ab anti-Toxocara, Ab anti-Schistosoma ELISA, Ab anti-Schistosoma ICT, Ab anti-Schistosoma WB, Ab anti-Fasciola hepatica, Ab anti-echinococco ELISA, Ab anti-echinococco IHA, Ab anti-cisticerco WB, Ab anti-dengue ELISA, Ab anti-dengue ICT, Ab anti-chikungunya , Ab anti-Zika, Ab anti-HTLV-I/II.
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