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________LABORATORY________
AGAR PLATE COPRO-CULTURE METHOD


Procedure useful for: Strongyloides stercoralis, Strongyloides fuelleborni, Necator americanus, Ancylostoma duodenale, Trichostrongylus spp, Ternidens deminutus and Oesophagostomum spp.


Agar Plate Copro-Culture. Microscopic observation.
Microscopic observation

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Strongyloides stercoralis

Agar Plate Copro-Culture: Strongyloides stercoralis first-stage rhabditoid/L1 larva (380x20 µm).
Strongyloides stercoralis first-stage rhabditoid/L1 larva (380x20 µm)

Agar Plate Copro-Culture. Strongyloides stercoralis first-stage rhabditoid/L1 larva: short buccal cavity.
Strongyloides stercoralis first-stage rhabditoid/L1 larva: short buccal cavity

Agar Plate Copro-Culture. Strongyloides stercoralis first-stage rhabditoid/L1 larva: genital primordium.
Strongyloides stercoralis first-stage rhabditoid/L1 larva: genital primordium

Agar Plate Copro-Culture. Strongyloides stercoralis third-stage filariform/L3 larva (600x16 µm).
Strongyloides stercoralis third-stage filariform/L3 larva (600x16 µm)

Agar Plate Copro-Culture. Strongyloides stercoralis third-stage filariform/L3 larva: notched tail.
Strongyloides stercoralis third-stage filariform/L3 larva: notched tail

Agar Plate Copro-Culture. Strongyloides stercoralis free-living female.
Strongyloides stercoralis free-living female

Agar Plate Copro-Culture. Strongyloides stercoralis free-living female.
Strongyloides stercoralis free-living female

Agar Plate Copro-Culture. Strongyloides stercoralis free-living female: cephalic part.
Strongyloides stercoralis free-living female: cephalic part

Agar Plate Copro-Culture. Strongyloides stercoralis free-living female: vulva.
Strongyloides stercoralis free-living female: vulva

Agar Plate Copro-Culture. Strongyloides stercoralis free-living female: vulva.
Strongyloides stercoralis free-living female: vulva

Agar Plate Copro-Culture. Strongyloides stercoralis free-living female: caudal end
Strongyloides stercoralis free-living female: caudal end

Agar Plate Copro-Culture. Strongyloides stercoralis free living male
Strongyloides stercoralis free-living male

Agar Plate Copro-Culture. Strongyloides stercoralis free-living male: spicule.
Strongyloides stercoralis free-living male: spicule

Agar Plate Copro-Culture. Free living females and one male of  Strongyloides stercoralis.
Free living-females and one male of Strongyloides stercoralis

Watch the movie! Agar Plate Copro-Culture: Strongyloides stercoralis free-living male and female in copula.
Strongyloides stercoralis free-living male and female in copula

Agar Plate Copro-Culture. Strongyloides stercoralis eggs (47,5x37,5 µm).
Strongyloides stercoralis eggs (47,5x37,5 µm)

Agar Plate Copro-Culture. Strongyloides stercoralis third-stage filariform/L3 larvae (630x16 µm).
Strongyloides stercoralis third-stage filariform/L3 larvae (630x16 µm)

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Strongyloides fuelleborni
Agar Plate Copro-Culture. Strongyloides fuelleborni egg (50x35 µm).
Strongyloides fuelleborni egg (50x35 µm)

Agar Plate Copro-Culture. Strongyloides fuelleborni third-stage filariform/L3 larva (620x16 µm).
Strongyloides fuelleborni third-stage filariform/L3 larva (620x16 µm)

Agar Plate Copro-Culture. Strongyloides fuelleborni third-stage filariform/L3 larva: notched tail.
Strongyloides fuelleborni third-stage filariform/L3 larva: notched tail

Agar Plate Copro-Culture. Strongyloides fuelleborni free living female (980x45 µm).
Strongyloides fuelleborni free-living female (980x45 µm)

Agar Plate Copro-Culture. Strongyloides fuelleborni free living female: vulva.
Strongyloides fuelleborni free-living female: vulva

Agar Plate Copro-Culture. Strongyloides fuelleborni free living female: vulva.
Strongyloides fuelleborni free-living female: vulva

Agar Plate Copro-Culture. Strongyloides fuelleborni free living male (570x30 µm).
Strongyloides fuelleborni free-living male (570x30 µm)

Agar Plate Copro-Culture. Strongyloides fuelleborni free living male: cephalic part.
Strongyloides fuelleborni free-living male: cephalic part

Agar Plate Copro-Culture. Strongyloides fuelleborni free living male: caudal end.
Strongyloides fuelleborni free-living male: caudal end

Agar Plate Copro-Culture. Strongyloides fuelleborni free living male: spicule.
Strongyloides fuelleborni free-living male: spicule

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Necator americanus
Agar Plate Copro-Culture. Necator americanus egg (40x60 µm).
Necator americanus egg (40x60 µm)

Agar Plate Copro-Culture. Necator americanus egg and larva.
Necator americanus egg and larva

Agar Plate Copro-Culture. Necator americanus first-stage rhabditoid/L1 larva (250-350x17 µm).
Necator americanus first-stage rhabditoid/L1 larva (250-350x17 µm)

Agar Plate Copro-Culture. Necator americanus first-stage rhabditoid/L1 larva: long buccal cavity.
Necator americanus first-stage rhabditoid/L1 larva: long buccal cavity
Agar Plate Copro-Culture. Necator americanus first-stage rhabditoid/L1 larva: genital primordium.
Necator americanus first-stage rhabditoid/L1 larva: genital primordium

Agar Plate Copro-Culture. Necator americanus third-stage filariform/L3 larva (600-700x25 µm).
Necator americanus third-stage filariform/L3 larva (600-700x25 µm)

Agar Plate Copro-Culture. Necator americanus third-stage filariform/L3 larva (600-700x25 µm).
Necator americanus third-stage filariform/L3 larva (600-700x25 µm)

Agar Plate Copro-Culture. Necator americanus third-stage filariform/L3 larva: cephalic part.
Necator americanus third-stage filariform/L3 larva: cephalic part

Agar Plate Copro-Culture. Necator americanus third-stage filariform/L3 larva: caudal end.
Necator americanus third-stage filariform/L3 larva: caudal end

Agar Plate Copro-Culture. Necator americanus third-stage filariform/L3 larva: cuticle.
Necator americanus third-stage filariform/L3 larva: cuticle

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Trichostrongylus spp

Agar Plate Copro-Culture. Trichostrongylus spp egg (75-95x40-50 µm).
 Trichostrongylus spp egg (75-95x40-50 µm)
Agar Plate Copro-Culture. Trichostrongylus spp first-stage rhabditoid/L1 larva (367x21,5 µm).
Trichostrongylus spp first-stage rhabditoid/L1 larva (367x21,5 µm)

Agar Plate Copro-Culture. Trichostrongylus spp first-stage rhabditoid/L1 larva: long buccal cavity.
Trichostrongylus spp first-stage rhabditoid/L1 larva: long buccal cavity

Agar Plate Copro-Culture. Trichostrongylus spp first-stage rhabditoid/L1 larva: genital primordium.
Trichostrongylus spp first-stage rhabditoid/L1 larva: genital primordium

Agar Plate Copro-Culture. Trichostrongylus spp first-stage rhabditoid/L1 larva: caudal end.
Trichostrongylus spp first-stage rhabditoid/L1 larva: caudal end

Agar Plate Copro-Culture. Trichostrongylus spp third-stage filariform/L3 larva (740x27,5 µm).
Trichostrongylus spp third-stage filariform/L3 larva (740x27,5 µm)

Agar Plate Copro-Culture. Trichostrongylus spp third-stage filariform/L3 larva: cephalic part.
Trichostrongylus spp third-stage filariform/L3 larva: cephalic part

Agar Plate Copro-Culture. Trichostrongylus spp third-stage filariform/L3 larva: intestine.
Trichostrongylus spp third-stage filariform/L3 larva: intestine

Agar Plate Copro-Culture. Trichostrongylus spp third-stage filariform/L3 larva: intestine.
Trichostrongylus spp third-stage filariform/L3 larva: intestine

Agar Plate Copro-Culture. Trichostrongylus spp third-stage filariform/L3 larva: caudal end.
Trichostrongylus spp third-stage filariform/L3 larva: caudal end
Copro-culture method
Centro per le Malattie Tropicali e Servizio autonomo di Epidemiologia e Laboratorio per le Malattie Tropicali. Mail: segreteria.malattietropicali@sacrocuore.it
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