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______LABORATORIO______

_______LABORATORY_______

SERVICE OF EPIDEMIOLOGY AND LABORATORY FOR TROPICAL DISEASES

The Service of Epidemiology and Laboratory for Tropical diseases cooperates very closely with the Centre for Tropical Medicine of the same Hospital.
Our Service performs laboratory tests for the Tropical Centre and for all the Departments of the Hospital, as well as for out-patients who are referred from general practitioners (prescription of the family doctor is required). In addition, the Service of Epidemiology and Laboratory for Tropical diseases offers a free telephone assistance for all practitioners: our specialists indicate the appropriate exams to perform and give explanation about the results.
Since many years, the Service has been organizing intensive training courses for people who intend to operate in Developing Countries as well as for italian sanitary personnel who wish to improve the knowledge in parasitology.
Our laboratory of tropical diseases performs microscopic identification of tropical and non tropical parasites as well as indirect (serological) and direct (antigenic) up-to-date diagnostic tests for specific infections. Most tests are commercially available; in some cases, laboratory-made tests are used (for example for the detection of anti-Strongyloides antibodies).

Tricuris trichiura egg (32-40 x 50-90 µm).
Plasmodium falciparum gametocyte.
Entamoeba histolytica trophozoite (20 x 60 µm).
Taenia solium rostellum.
Mammomonogamus laryngeus adults (female: 5-20 mm; male: 2-6 mm).
Schistosoma mekongi egg (39-66 x 51-78 µm)
Plasmodium malariae trophozoite.Endolimax nana cyst (4-8 µm).Myiasi. Dermatobia hominis (1-2.5 cm).Bertiella sp proglottid (one segment 0.68 x 1.10 cm).Lice. Phthirus pubis female (1.5-2 mm).Trypanosoma congolense trypomastigote.Echinococcus sp hooklets (18-35 µm).Mesocestoides sp egg (19-24 µm).Microgametocyte of Plasmodium vivax.Tricuris vulpis egg (32-40 x 72-90 µm).Iodamoeba butschlii cyst (5-20 µm).Balantidium coli trophozoite (20-200 µm).Babesia bigenima.Diphyllobothrium latum egg (40-50 x 58-75 µm).
Entamoeba coli cyst (15-20 µm).
Tricuris trichiura female (35-50 µm).
Plasmodium falciparum trophozoite.
Cyclospora cayetanensis oocyst (8-10 µm).
Trypanosoma rangeli trypomastigote.
Iodamoeba butschlii cyst (5-20 µm).
Mite. Demodex folliculorum (50-300 µm).
Strongyloides stercoralis first-stage larva (14-20 x 180-380 µm).
Plasmodium malariae trophozoite.Isospora belli immature oocyst (10-19 x 20-33 µm).
Entamoeba histolytica trophozoite (20-60 µm).
Hymenolepis nana egg (30-47 µm).Plasmodium vivax trophozoite.Hard tick. Ixodes ricinus female (some mm to more then 1 cm).Strongyloides fuelleborni egg (30-34 x 55-58 µm).Trypanosoma lewisi trypomastigote.Entamoeba coli trophozoite (15-50 µm).Isospora belli mature oocyst (10-19 x 20-33 µm).Enterobius vermicularis egg (20-30 x 50-60 µm).Plasmodium ovale trophozoite.Echinococcus sp protoscolece.Loa loa microfilaria (6.0-8.5 x 231-250 µm).Ascaris lumbricoides female (3-6 x 20-35 cm).Chordodes sp (hair worm) - (da 10 a 50 cm).Taenia sp egg (31-43 µm).Endolimax nana trophozoite (4-10 µm).Trypanosoma brucei gambiense/rhodesiense trypomastigote.Dientamoeba fragilis trophozoite (5-20 µm).Wuchereria bancrofti microfilaria (7.5-10 x 244-296 µm).Schistosoma haematobium miracidiumTaenia saginata proglottid (5-7 x 18-20 mm).Ascaris lumbricoides egg (35-50 x 55-75 µm).Plasmodium falciparum trophozoiteTrypanosoma cruzi trypomastigote.Enterobius vermicularis female (0.3-0.5 x 8-13 mm).Cryptosporidium sp oocyst (4-6 µm).Leishmania sp amastigotes (1-2 x 1-5µm).Brugia malayi microfilaria (5-6 x 177-230 µm).Iodamoeba butschlii trophozoite (8-20 µm).Dioctophyme renale egg (39-46 x 60-80 µm).Mansonella perstans microfilaria (4 x 190-200 µm).Trichostrongylus sp egg (40-50 x 75-95 µm).Entamoeba histolytica/E. dispar cyst (10-20 µm).Schistosoma mansoni egg (45-70 x 114-175 µm).Micro and macrogametocytes (Plasmodium falciparum).Strongyloides stercoralis female (30-40 µm x 2.1-2.7 mm).Giardia intestinalis cyst (8-19 µm).Loa loa adults (female 0.5 x 50-70 mm, male 0.35-0.43 x 30-35).Schistosoma intercalatum egg (50-85 x 140-240 µm).Fasciola hepatica adult (13 x 30 mm). Toxocara sp egg (75 x 80-85 µm).Strongyloides stercoralis filariform larva (16 x 600 µm).Schistosoma japonicum egg (55-65 x 70-100 µm).Giardia intestinalis trophozoite (10-20 µm).Lice. Pediculus humanus capitis female (3-4 mm).Paragonimus westermanni egg (45-70 x 80-120 µm).Giardia intestinalis trophozoite (10-20 µm).
Physaloptera sp egg (44-65 µm).
Ascaris lumbricoides infertile egg (43-47 x 85-95 µm).Hymenolepis diminuta egg (78-85 µm).Blastocystis hominis (5-15 µm).Anatrichosoma sp egg (58 x 78 µm).Capillaria philippinensis egg (21 x 36-45 µm).Malaria microgametocyte in exflagellation.Bertiella sp egg (33-43 x 38-46 µm).Plasmodium ovale immatute schizont.Sarcocystis hominis oocyst (15-20 x 15-19 µm).Metagonimus yokogawi egg (15-17 x 20-30 µm).Dicrocoelium dendriticum adult (1.5-2.5 x 5-15 mm).Cyclospora sp oocyst (8-10 µm).Chilomastix mesnili trophozoite (6-24 µm).Dicrocoelium dendriticum egg (22-30 x 38-45 µm).Entamoeba coli cyst (15-20 µm).Gongylonema sp egg (25-37 x 40-50 µm).Mite egg (85-100 x 130-145 µm).Clonorchis sinensis egg (12-19 x 19-35 µm).Fasciola buski egg (63-90 x 130-150 µm).Plasmodium vivax mature schizont.Capillaria hepatica egg (30-35 x 51-67 µm).Entamoeba coli trophozoite (15-50 µm).Ascaris lumbricoides male (2-4 x 15-31 mm).Inermicapsifer sp egg (40 x 75 µm).Diplogonoporus grandis egg (33-48 x 60-83 µm).Dipylidium caninum eggs (egg 25-40 µm). Babesia microti.Hard tick. Rhipicephalus sanguineus male (about 3 mm).Fasciola hepatica egg (63-90 x 130-150 µm).Macracanthorynchus birudinaceus egg (50 x 80-100 µm).Trypanosoma evansi trypomastigote.Schistosoma haematobium egg (40-70 x 112-170 µm).Flea. Tunga penetrans

 

  Intestinal parasites examination  
  • Copro-parasitological tets
  • Permanent Staining for intestinal protozoa
  • Scotch-Tape test
  • Cryptosporidium detection

  Urine parasites examination  

  • Uro-parasitological test

  Blood protozoa examination  

  • Malaria QBC. FD method
  • Malaria in blood film. Thick and thin film examination
  • Leishmania in bone marrow. Microscopic examination
  • Trypanosoma HCT. Capillary hematocrit method
  • Trypanosoma
    •  QBC. FD method

  Cutaneous protozoa examination  

  • Leishmania in skin. Microscopic examination

  Blood helminths examination  

  • Microfilariae detection. Concentration technique. 
  Cutaneous helminths examination  
  • Skin-snip  test

  Lungs and polmonary vessels parasites  

  • Sputum examination

  Serological tests  

  • Ab anti-malaria IgG. IIF method
  • Ab anti-amoeba IgG. IIF method
  • Ab anti-Strongyloides stercoralis IgG. IIF method
  • Ab anti-Schistosoma sp IgG. IIF method
  • Ab anti-Leishmania spp (visceral) IgG. IIF method
  • Ab anti-Trypanosoma b. gambiense IgG. IIF method
  • Ab anti-Trypanosoma cruzi AgLis IgM/IgG. ELISA method
  • Ab anti-Trypanosoma cruzi AgRic IgM/IgG. ELISA method
  • Ab anti-Trypanosoma cruzi IgM/IgG. ICT method
  • Ab anti-Toxocara sp IgG. ELISA method
  • Ab anti-Echinococcus spp. IgG. ELISA method
  • Ab anti-cysticercus IgG. ELISA method
  • Ab anti-cysticercus IgG. Western blot method
  • Ab anti-dengue IgM/IgG. ELISA method
  • Ab anti-dengue IgM/IgG. ICT method
  • Ab anti-chikungunya IgM. ICT method
  Antigenic tests  
  • Malaria antigen. ICT method
  • Entamoeba histolytica/E. dispar antigen. ELISA method
  • Giardia intestinalis antigen. ELISA method
  • Entamoeba histolytica antigen. ELISA method
  • Dengue antigen. ICT method

  Copro-culture  

  Identification  

  • Endoparasites and ectoparasites identification


  • Ab anti-Anisakis specific IgE. RAST methond


  • Ab anti-filaria IgG. ELISA methond (I.S.S. - Roma)
  • Ab anti-Trichinella spiralis IgG. ELISA methond (I.S.S. - Roma)
  • Leishmania spp culture (I.S.S. - Roma)
  • Trypanosoma cruzi PCR (Barcelona - Spain)
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Centro per le Malattie Tropicali e Servizio autonomo di Epidemiologia e Laboratorio per le Malattie Tropicali. Mail: segreteria.malattietropicali@sacrocuore.it
Ospedale classificato ed equiparato Sacro Cuore - Don Calabria Via don A. Sempreboni, 5 - 37024 Negrar, Verona (Italy)
P.IVA 00280090234 - Work tel. (+39) 045 6013111 - fax (+39) 045 7500480 - pronto soccorso 045 6013222 - EMERGENZE: 118
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