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______LABORATORIO______

_______LABORATORY_______

SERVICE OF EPIDEMIOLOGY AND LABORATORY FOR TROPICAL DISEASES

The Service of Epidemiology and Laboratory for Tropical diseases cooperates very closely with the Centre for Tropical Medicine of the same Hospital.
Our Service performs laboratory tests for the Tropical Centre and for all the Departments of the Hospital, as well as for out-patients who are referred from general practitioners
(prescription of the family doctor is required). In addition, the Service of Epidemiology and Laboratory for Tropical diseases offers a free telephone assistance for all practitioners: our specialists indicate the appropriate exams to perform and give explanation about the results.
Since many years, the Service has been organizing intensive training courses for people who intend to operate in Developing Countries as well as for italian sanitary personnel who wish to improve the knowledge in parasitology.
Our laboratory of
tropical diseases performs microscopic identification of tropical and non tropical parasites as well as indirect (serological) and direct (antigenic) up-to-date diagnostic tests for specific infections. Most tests are commercially available; in some cases, laboratory-made tests are used (for example for the detection of anti-Strongyloides antibodies).

 



Direct examination for intestinal parasites
  • Faecal microscopy
  • Permanent staining for intestinal protozoa
  • Adhesive tape test (scotch-tape test)
  • Cryptosporidium sp

 Direct examination for urine parasitess

  • Urine microscopy

Direct examination for blood protozoa

  • Malaria QBC. FD Method
  • Malaria microscopy. Thick and thin film examination
  • Leishmania spp in bone marrow. Microscopic examination
  • Trypanosoma spp. Microhaematocrit tube method
  • Trypanosoma spp. QBC. FD Method

 Direct examination for cutaneous protozoa

  • Leishmania spp. Microscopic examination

Direct examination for blood helminths

  • Microfilariae. Concentration technique. 
Direct examination for cutaneous helminths
  • Skin-snip 
Serological tests
  • Ab anti-malaria IgG. IIF method
  • Ab anti-amoeba IgG. IIF method
  • Ab anti-Strongyloides stercoralis IgG. IIF method
  • Ab anti-Schistosoma sp IgG. IIF method
  • Ab anti-Leishmania spp (visceral) IgG. IIF method
  • Ab anti-Trypanosoma b. gambiense IgG. IIF method
  • Ab anti-Trypanosoma cruzi IgM/IgG. ELISA method
  • Ab anti-Trypanosoma cruzi IgM/IgG. ICT method
  • Ab anti-Toxocara sp IgG. ELISA method
  • Ab anti-Echinococcus spp. IgG. ELISA method
  • Ab anti-cysticercus IgG. ELISA method
  • Ab anti-cysticercus IgG. Western blot method
  • Ab anti-dengue IgM/IgG. ELISA method
  • Ab anti-dengue IgM/IgG. ICT method
  • Ab anti-chikungunya IgM. ICT method
Antigenic tests
  • Malaria antigen. ICT method
  • Giardia intestinalis antigen. ELISA method
  • Entamoeba histolytica/E. dispar amoebic antigen. ELISA method
  • Entamoeba histolytica amoebic antigen. ELISA method     

 Faecal culture method

  • Faecal culture for Strongyloides stercoralis and other intestinal helminths. Agar plate culture method

 Identification

  • Endoparasites and ectoparasites
 

    • Ab anti-filaria IgG. ELISA methond (I.S.S. - Roma)
    • Ab anti-Trichinella spiralis IgG. ELISA methond (I.S.S. - Roma)
    • Leishmania spp culture (I.S.S. - Roma)
    • Trypanosoma cruzi PCR (Barcelona - Spain)
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Centro per le Malattie Tropicali e Servizio autonomo di Epidemiologia e Laboratorio per le Malattie Tropicali. Mail: segreteria.malattietropicali@sacrocuore.it
Ospedale classificato ed equiparato Sacro Cuore - Don Calabria Via don A. Sempreboni, 5 - 37024 Negrar, Verona (Italy)
P.IVA 00280090234 - Work tel. (+39) 045 6013111 - fax (+39) 045 7500480 - pronto soccorso 045 6013222 - EMERGENZE: 118
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